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KMID : 0644120050180010015
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Journal of Oriental Gynecology
2005 Volume.18 No. 1 p.15 ~ p.28
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Kung-Kyung-IlHo-Jeon on Induced Apoptosis in Human CervicalCarcinoma HeLa Cells
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Kang Young-Keum
Choi Chang-Min
Cho Han-Baek
Yoo Sim-Keun
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Abstract
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To address the ability of Kung-Kyung-Ilho-Jeon(KK) to induce cell death, we investigated the effect of KK on cell viability. Forty-eight hours later, loss of viability occurred following KK exposure in a dose-dependent manner. The treatment of KK, a commonly used herb formulation in Korea and China, caused a decrease in cell viability. KK also resulted in apoptotic morphology a brightly blue-fluorescent condensed nuclei by Hoechst 33258-staining, and reduction of cell volume. Our results show that KK induces caspase-3 and -9 activation in a time-dependent manner. In addtion, the translocation of cytochrome c release into cytoplasm has been observed under the presence of 5 ㎎/㎖ KK. The subsequent loss of mitochondria membrane potential is collapsed by the addition of KK. Our immunoblotting data show that PARP, a well known caspase-3 and -6 substrate, is cleaved by KK. We show that a pro-apoptotic protein, Bax is increased in the presence of KK but that the amount of Bcl-2 is not changed. We suggest that Bax, a critical protein which can regulate channel of mitochondria to release cytochrome c, is a key protein in KK-induced apoptosis of Hela human cervical carcinoma cells
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KEYWORD
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Kung-Kyung-Ilho-Jeon(KK), Bax, mitochondria membrane potential, apoptosis, PARP, HeLa cells
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